动物造模,鹌鹑痛风模型,药效评价 zi-bio 2024-11-20 860

　　Objective: To simulate the induction of gout in Difarc quails through a high protein and high calcium diet in clinical practice, and to preliminarily explore the method of establishing a quail gout model, providing a basis for the study of gout pathogenesis and drug screening for prevention and treatment

　　Method: Thirty six healthy 30 day old male Difak quails were randomly divided into three groups based on body weight and blood uric acid levels, namely the control group, model I group, and model II group, with 12 quails in each group. The control group quails were fed a basic diet, while the model group quails were fed a high protein and high calcium diet (with a crude protein content of 29.5% and a calcium content of 7.8% in the diet). The control group and model I group drank water freely. The model II group was restricted to drinking water for 4 hours per day, and the experiment lasted for 50 days. The serum and fecal uric acid levels of quails were measured, while renal function was measured and histological and pathological sections were observed

　　Result: A high protein and high calcium diet can significantly increase the serum uric acid level in quails; The level of fecal and urinary uric acid significantly decreased; Elevated levels of serum creatinine and urea nitrogen; Causing significant deposition of urate in quail kidneys. A high protein and high calcium diet combined with restricted drinking water can exacerbate elevated blood uric acid levels and renal urate deposition, leading to gout symptoms such as joint swelling and deformation in some quails

　　Conclusion: Feeding a high protein and high calcium diet with limited water intake can establish a gout quail model, and its pathogenesis is more consistent with clinical gout etiology, pathogenesis, and clinical manifestations, providing reference for the basic research and drug development of gout