动物造模,糖尿病 zi-bio 2024-04-28 843

　　Objective: To explore a stable and reliable method for establishing a mouse model similar to gestational insulin resistance.

　　Method: Sixty SPF grade 5-week-old KM mice were randomly divided into a high-fat diet group and a general diet group. The high-fat diet group was fed with high-fat diet for 4 weeks, and the cages were closed in a 1:1 ratio between male and female. The vaginal plug was located in the vagina of the female mice on the first day of pregnancy. After successful pregnancy, intraperitoneal injections were administered at intervals of 30 mg/kg for 24 hours, with a total of 3 injections. The control group was injected with an equal amount of citric acid buffer (0.1 mol/L, pH=4.2). On the 3rd, 7th, 14th, and 19th day after successful modeling, random blood glucose, body weight, as well as 24-hour water and food intake of mice were recorded. ELISA is used to detect the concentrations of INS, ADP, LEP, and CRP factors in serum.

　　Result: Pregnant mice showed significant symptoms of increased water intake, food intake, and urine output after successful modeling. There were significant differences in water intake and food intake compared to the control group (P<0.01), with="" blood="" glucose="" levels="" in="" the="" modeling="" group="">11.1 mmol/L, significantly higher than those in the control group. GDM group INS (1.50 ± 0.25) Mu/L, ADP (0.65 ± 0.13) μ G/L, LEP (1.60 ± 0.12) μ G/L, CRP (37.54 ± 4.70) μ G/L, significantly different from the control group (P<0.01). After childbirth, the blood sugar of the mice returned to normal levels.

　　Conclusion: The GDM model can be established by high-fat diet combined with multiple induction of low-dose STZ, which is consistent with the characteristics of pathological insulin resistance in human gestational diabetes.