动物造模,急性肝损伤动物模型 zi-bio 2024-05-06 617

　　Objective: To explore the modeling elements of various acute liver injury animal models, and provide reference and suggestions for the establishment and evaluation of acute liver injury animal models.

　　Method: Retrieve animal experimental literature on acute liver injury from 2002 to 2022 in databases such as CNKI, WanFang, VIP, Yigle, and PubMed. Summarize the animal types, positive control drugs, modeling methods, modeling drugs and dosage, administration time, and detection indicators of acute liver injury animal models mentioned in the literature. Analyze the results using Excel, SPSS Modeler 18.0, and Cytoscape 3.8.2.

　　The database included a total of 896 articles, with male KM mice being the most commonly used animal model for acute liver injury; The modeling methods mainly include four categories: chemical liver injury, alcoholic liver injury, drug-induced liver injury, and immune liver injury. The corresponding main modeling methods are: ① intraperitoneal injection of 10.0mL/kg0.1% CCl4 vegetable oil 24 hours before the experiment; ② 16 hours before the experiment, 12.0mL/kg50.0-56.0% ethanol was orally administered; ③ Intraperitoneal injection of APAP 300mg/kg 24 hours before the experiment; ④ ConA20mg/kg was injected into the tail vein 8 hours before the experiment. The evaluation of the model is based on liver pathological indicators as the gold standard, combined with biochemical indicators such as serum ALT and AST, liver tissue homogenate SOD and MDA content and activity as direct indicators.

　　Conclusion: The causes of acute liver injury vary in clinical practice. When preparing animal models of acute liver injury, corresponding modeling methods should be selected based on specific research content and characteristics.