动物造模 zi-bio 2024-05-06 465

　　Purpose: To establish a sterile APPswe/PS1 using a cesarean section purification method Δ E9 (PAP) dual transgenic mouse model and preliminary observation of plaque deposition in the animal brain, providing a new animal model for studying the relationship between gut microbiota and Alzheimer's disease

　　Method: Positive PAP male heterozygous mice were selected to mate with multiparous C57 wild-type female mice at a ratio of 1:2. Pregnant female mice underwent cesarean section surgery in an ultra clean workbench, and sterile ICR mice were used as milk substitutes. After surgery, sterile status testing was conducted every month; PCR method was used to detect the genotype of PAP offspring obtained from cesarean section; Immunohistochemical methods were used to quantitatively detect changes in plaques in the brain of 9-month-old PAP mice

　　Result: Twelve cases of cesarean section surgery were performed, and 66 offspring were obtained. The cesarean section survival rate and weaning survival rate were 95.45% (63/66) and 95.24% (60/63), respectively. After purification, the sterile status was tested according to national standards and all were qualified. Immunohistochemical results showed that plaques in the hippocampus of 9-month-old sterile PAP mice were reduced compared to SPF grade animals of the same age

　　Conclusion: The bacterial community carried by PAP mice was removed through cesarean section purification technology, and plaques in the brain of 9-month-old sterile PAP mice were reduced