动物造模,肺成纤维细胞模型 zi-bio 2024-05-06 465

　　Objective: To explore the feasibility of establishing an experimental model of CA16 infection in tree shrew lung fibroblasts

　　Method: TSLF was infected with enterovirus CA16, and human lung fibroblasts (KMB-17) were used as controls. The cellular lesions were observed under the microscope, and the expression of virus protein and infection related receptor SCARB2 protein was observed through indirect immunofluorescence assay. The viral load was detected by real-time fluorescence quantitative PCR using probe method β- Using Actin as an internal reference dye, real-time fluorescence quantitative PCR was used to detect the relative expression level of receptor SCARB2 gene. Combined with gene sequence alignment, its correlation with infection was analyzed

　　Result: CA16 infection with TSLF can cause significant cellular damage, and immunofluorescence experiments show that the virus and receptor SCARB2 protein can be detected. Infection with TSLF at a ratio of 100TCID50/105 cells can reach the highest viral load after about 48 hours. The SCARB2 gene has high expression related to infection, and its gene sequence also has high homology with humans

　　Conclusion: CA16 can infect TSLF, and tree shrew SCARB2 can participate in the infection