动物造模 zi-bio 2024-05-06 629

　　Objective: To observe the efficiency and safety of establishing an atrial fibrillation model by innovatively using fine electrode wires, advanced catheter manipulation systems, and implantable electrocardiogram monitors in traditional high-frequency atrial pacing modeling methods

　　Method: Twelve adult beagle dogs were selected and randomly divided into a blank control group (6 dogs) and an atrial fibrillation model group (AF group, 6 dogs). The AF group used the SelectSecure system to implant a thin bipolar solid electrode wire with a diameter of only 4.1 Fr, 3830 actively fixed electrodes, connected to a dedicated atrial fibrillation model pacemaker to establish an atrial high-frequency pacing system. After surgery, an atrial fibrillation model was established using fast atrial pacing in AOO pacing mode, and the occurrence of atrial fibrillation was tracked in real-time using an implanted electrocardiogram monitor, RevealLINQ tracker. The experimental dogs were examined by echocardiography using a heart color ultrasound scanner with a probe radiofrequency of 2.5 MHz. Measure the end systolic atrial area of the left and right atria through the four chambers of the apex. Observe the morphology of the left atrial tissue under light and electron microscopy

　　Result: Among the 12 experimental dogs, 2 of them died in the AF group during the modeling process, and the remaining 10 experimental dogs completed the experiment. The time to successfully establish the atrial fibrillation model was (10.63 ± 2.13) weeks; When the atrial fibrillation model was successfully established, in AOO mode, the atrial stimulation frequency of the high-frequency atrial fibrillation model pacemaker was (588.75 ± 11.26) times/min. The implanted electrocardiogram monitor used could accurately and efficiently track and record the atrial fibrillation load in the AF group. After the successful creation of the atrial fibrillation model, the left atrial surface area significantly increased compared to before modeling [(8.20 ± 0.83) cm2 compared to (3.80 ± 0.08) cm2, P<0.05]; The area of the right atrium increased compared to before modeling [(4.52 ± 0.44) cm2 compared to (2.75 ± 0.96) cm2, P<0.05]; The left atrial area in the AF group increased compared to the control group [(8.20 ± 0.83) cm2 compared to (3.72 ± 0.15) cm2, P<0.05]; The area of the right atrium increased compared to the control group [(4.52 ± 0.44) cm2 compared to (2.78 ± 0.18) cm2, P<0.05]. Morphological observation of left atrial tissue slices in atrial fibrillation model dogs and blank control experimental dogs showed that the atrial structure in atrial fibrillation experimental dogs underwent remodeling

　　Conclusion: The use of high-frequency atrial pacing can successfully establish a stable model of atrial fibrillation. The implantation of 3830 bipolar solid atrial pacing electrodes using the SelectSecure system can accurately implant the electrodes and improve the success rate of electrode implantation in experimental dogs. The use of the implantable electrocardiogram monitor RevealLINQ for real-time dynamic monitoring of atrial fibrillation load can improve experimental monitoring efficiency, efficiency, and accuracy