动物造模,动脉粥样硬化 zi-bio 2024-05-06 601

　　Objective: To construct a rat model of atherosclerosis combining disease and syndrome, and to provide an evaluation tool for the pharmacodynamics research of corresponding Chinese herbal formulas

　　Methods: According to the relevant data and literature research, the diagnostic and therapeutic indexes for evaluating the rat model of atherosclerosis (qi stagnation and blood stasis syndrome) were selected, and Simvastatin tablets and Tongmai granules were used as the anti evidence drugs. Combined with the modern research on the evolution law of the etiology and pathogenesis of atherosclerosis and the pathogenesis, it is proposed to use ice water bath, intragastric fat emulsion and tail vein injection of bovine serum albumin to prepare the disease syndrome combination model of atherosclerosis (qi stagnation and blood stasis syndrome) in rats. During the experiment, the symptoms and signs of rats in each group were investigated and scored. After 8 weeks of modeling, the four levels of blood lipids and coagulation of rats in each group were detected, and the pathological changes of ascending aorta were observed to form the model Pre test. After successful modeling, use anti evidence drugs to intervene, and measure the four levels of blood lipid and blood coagulation of rats, and detect the pathological changes of ascending aorta and liver tissue

　　Results: After 21 weeks of modeling, compared with the normal group, the symptoms and signs of the model group rats changed significantly, the blood lipid level and fibrinogen content were significantly increased, and the activated part of prothrombin time was significantly reduced. Lipid deposition appeared under the intima of the ascending aorta, and hepatocytes appeared vacuoles and degeneration, basically in line with the clinical manifestations of atherosclerosis (qi stagnation and blood stasis syndrome). After drug intervention, compared with the model group, the blood lipid level and fibrinogen content of the drug anti syndrome group rats were significantly reduced, and the intima of the ascending aorta and liver tissue were not significantly abnormal

　　Conclusion: The combined induction method of ice water bath, intragastric fat emulsion and tail vein injection of bovine serum albumin for 21 weeks can establish a rat model simulating clinical atherosclerosis (qi stagnation and blood stasis syndrome), and has the characteristics of high modeling rate, similar to clinical symptoms and signs, which will provide evaluation for the pharmacodynamics research of anti atherosclerosis (qi stagnation and blood stasis syndrome) traditional Chinese medicine prescriptions