动物造模 zi-bio 2024-05-06 564

　　Objective: To select a stable expression of luciferase reporter gene in human liver cancer cell line (HCCLM3 Luc) and construct a nude mouse model of in situ cancer bearing human liver cancer cell line

　　Method: A live imaging system was used to quantitatively analyze the fluorescence intensity expression of HCCLM3 Luc with different cell numbers, and the luciferase activity of each cell number was detected to explore the linear correlation between the photon quantity produced by HCCLM3 Luc luciferase and the number of tumor cells. HCCLM3 Luc tumor tissue was inoculated into the liver lobe in situ and HCCLM3 Luc cell suspension was injected into the tail vein to construct a nude mouse in situ cancer model of human liver cancer; Compare the feasibility of two methods for constructing in situ nude mouse models of human liver cancer cell lines and optimize the key factors in model construction

　　Result: The number of HCCLM3 Luc cells was positively correlated (correlation coefficient R2=0.9989, linear equation: Y=1155.8X+1 × 106), with an average ROI value of approximately 140 photons/s per cell. The in situ tumor formation rate in the liver lobe group [(82.5 ± 7.2)%] was significantly higher than that in the tail vein injection group [(34.1 ± 13.2)%]

　　Conclusion: This study constructed a bioluminescent human liver cancer cell line in situ nude mouse model, which can monitor tumor growth in real-time using a small animal imaging instrument