动物造模,胃肠道功能紊乱,药效评价 zi-bio 2024-05-16 602

　　1. Animal modeling materials: healthy adult rats, male; Medication: Risepin.

　　2. Moulding method: The animal in the modeling group was intraperitoneally injected with 0.5mg/(kg · d) of reserpine for 14 consecutive days; The control group was intraperitoneally injected with 0.5mg/(kg · d) of physiological saline.

　　3. Modeling principle: Resveratrol causes gastrointestinal dysfunction in animals.

　　4. Changes after modeling: Within 24 hours after injection of reserpine, the animals in the modeling group experienced fecal irregularity, varying degrees of diarrhea, perianal contamination, and decreased appetite. On the 6th day, loose stools were particularly severe, and on the 8th day, loose stools stopped. Feces were sticky and unpleasant, and even did not defecate. Anorexia and weight loss worsened day by day.

　　Under naked eye observation, the gastric mucosa of the modeling group rats was pale in color, and the intestinal smoothness was poor. The color was light, and the small intestine was significantly inflated. The intestinal wall became thinner, but no mucosal defects or ulceration were observed. The control group showed no abnormalities in the gastric mucosa.

　　Electron microscopy observation shows that the gastrointestinal mucosal epithelium of the model animal is necrotic and shedding, with a large number of autophagic vesicles in the epithelial cells, glandular atrophy, submucosal edema, loose cell arrangement, and sparse and disordered arrangement of intestinal mucosal microvilli, with varying lengths.