动物造模,胆结石,药效评价 zi-bio 2024-05-16 598

　　1. Animal modeling materials: Healthy adult dogs or rabbits, regardless of gender; Medications: Anesthetics, sterilized roundworm fragments (or human gallstones, knots, rubber, etc.).

　　2. Method of modeling: Under sterile conditions, open the abdomen and expose the gallbladder. Make a small incision at the bottom of the gallbladder, and implant sterilized roundworm fragments (or human gallstones, knots, rubber, etc.) into the gallbladder. Then, suture the gallbladder with a purse string. Implants should be dried to a constant weight beforehand, and their size should be measured for weighing and length comparison before and after the experiment. It can be examined by cesarean section 2-3 months later.

　　3. Principle of modeling: Foreign body implantation leads to animal gallstones.

　　4. Changes after modeling usually occur in the early stages of inflammation after the implantation of foreign objects in the gallbladder of rabbits, with an increase in mucus in the bile and sometimes even the formation of mucus clusters. Within 2-3 months, most black fine sand can be seen in the gallbladder, and foreign objects can be enveloped by dark green gallstones. If multiple foreign objects are implanted, they can sometimes be bonded into a large lump, and this model can be used for stone prevention and dissolution research with traditional Chinese and Western medicine or other treatment measures.