动物造模,肾阴虚证,药效评价 zi-bio 2024-05-17 447

　　(1) Reproduction method: Adult mice were used to prepare S180 tumor cells into 1g/4ml physiological saline cell suspension, and 0.2ml of each was inoculated subcutaneously in the armpit of the right forelimb. Starting from the next day, T4120mg and 0.8mg/(kg body weight · d) of reserpine were administered by gavage for 5 days. Observe the general condition of animals.

　　(2) The characteristics of the model are that animals have two eyes that are not open or blind, sometimes their whole body shakes, they are not active, and then they become thin, do not eat, and have slow reactions.

　　(3) Compared with medical subcutaneous tumor models, the syndrome manifestations vary at different stages of the disease, with fewer and simpler early syndromes, mainly characterized by Qi deficiency syndrome; The late stage syndrome is complex and can manifest as Yin deficiency syndrome, Yin Yang deficiency syndrome, etc. Therefore, the term S180 sarcoma kidney yin deficiency animal model in this method is not appropriate.