动物造模,急性坏死性胰腺炎,药效评价 zi-bio 2024-05-17 543

　　1. Animal modeling materials: Adult hybrid dogs, 10-15kg, regardless of gender; Medications: trypsin, pentobarbital.

　　2. Method of animal modeling: Fasting for 12 hours before the animal experiment, water cannot be avoided. After anesthesia, the duodenum is found through a midline incision in the upper abdomen. The duodenal wall is cut near the opening of the pancreatic duct, and a thin plastic tube is placed inside the main pancreatic duct. Then, 2-3 ml (0.3 ml/kg) of trypsin and dog's own bile mixture is injected inward, with a formula of 1 ml of bile+4 ml of trypsin solution containing 4 mg of trypsin. After being retained for 5 minutes, the plastic tube is removed, and the duodenal wall is sutured with a pouch. The abdomen is closed.

　　3. Principle of modeling: Pancreatobiliary obstruction, bile reflux, or duodenal reflux can interfere with the normal function of glandular cells, leading to activation of trypsinogen by intracellular lysosomal enzymes, resulting in pancreatitis.

　　4. Changes after modeling: A significant increase in serum amylase was observed 1 hour after surgery, lasting for 24 hours and reaching its peak at 6 hours; The gross and microscopic pathological changes of the pancreas show typical necrosis, bleeding, and infiltration of inflammatory cells, indicating that this method can successfully induce a canine model of acute necrotizing pancreatitis. Pathological changes: Extreme swelling of pancreatic tissue is visible to the naked eye, necrosis appears in the pancreatic parenchyma and submucosa, saponification spots form on the mesentery, and a large amount of bloody ascites and intestinal distension are seen in the abdominal cavity; Under the light microscope, there is swelling of pancreatic acinar cells, interstitial edema, bleeding, infiltration of inflammatory cells, microvascular congestion, and a few cases of microthrombus formation. Necrosis of acinar cells and adipocytes.

　　5. Precautions: Surgical instruments should be strictly disinfected to prevent surgical infections, surgical trauma should be minimized, and sterile operations should be strictly carried out.