动物造模,人胃癌裸鼠原位移植转移,药效评价 zi-bio 2024-05-20 769

　　【 Modeling Mechanism 】 The human gastric cancer nude mouse in situ transplantation model often uses subcutaneous transplantation method, and the transplanted tumor generally does not metastasize. In order to establish an in situ transplantation model for gastric cancer invasion and metastasis in nude mice, fresh human gastric cancer tissue was used for in situ transplantation in nude mice to establish high metastasis models for human gastric adenocarcinoma, squamous adenocarcinoma, squamous cell carcinoma, and in situ transplantation in nude mice, making the metastasis model similar to clinical patients.

　　【 Modeling Method 】 Sterile resection of human gastric cancer primary lesion tissue was placed in RPMI 1640 culture medium, and the tumor tissue was cut into small pieces of 1 cubic millimeter tissue for transplantation. The recipient nude mice were anesthetized and underwent microsurgical procedures. A midline incision was made in the upper abdomen, and a 2mm long incision was made along the lesser curvature of the gastric antrum, deep into the mucosal layer. A 1 cubic millimeter tumor was placed in the mucosal layer of the gastric wall using a non-invasive suture, and then the surgical incision was sutured. Continue to raise after surgery.

　　【 Model characteristics 】 Nude mice bearing tumors are generally on the brink of death due to exhaustion in the 5th to 6th week. The gastric wall is infiltrated by tumor tissue in various layers, often infiltrating the serosal layer and accompanied by visible lymph node and liver metastasis.

　　The number of tumor cells should be maintained at 100000 or more, and the injection site should reach deep into the mucosal layer. The surgical process should pay attention to sterile operation to prevent infection.

　　The evaluation and application of the model: Human gastric cancer transplanted tumor grows invasively in the gastric wall of nude mice, invading and destroying the tissue structure of each layer of the gastric wall, and directly invading adjacent organs and tissues. It then metastasizes to the liver, lungs, spleen, kidneys, etc. through blood circulation. Lymphatic metastasis to the mucosa and submucosal lymphoid plexus of the stomach. Local and distant lymph nodes are often accompanied by pyloric obstructive ascites. The human gastric cancer nude mouse orthotopic transplantation model is mainly used to study the pathogenesis and treatment of gastric cancer, and has significant applications in the basic and clinical medical fields of gastric cancer.