动物造模,门静脉高压,药效评价 zi-bio 2024-05-20 724

　　(1) Reproduction method: Adult experimental hybrid dogs weighing around 15kg were fasted before surgery and anesthetized by intravenous injection of 3% pentobarbital sodium at a dose of 30mg/kg body weight. The inferior vena cava and portal vein were anastomosed laterally with a anastomotic length of 2.5-3.0cm. A steel ring containing an agar ring was placed in the portal vein above the anastomotic site for fixation. After absorbing water, agar continuously expands and gradually blocks the portal vein; Double ligate the vena cava above the anastomotic site to completely inject blood into the portal vein system. After 3-6 weeks, all models were formed and there was no death. The model showed a significant increase in portal vein pressure, varying degrees of varicose esophageal and gastric venous plexus, and no significant changes in liver histology.

　　(2) The characteristics of the model include simple method, good reliability, low mortality rate, and a modeling rate of up to 100%.

　　(3) The comparative medicine model is a portal vena cava anastomosis and partially narrowed portal vein trunk model, which has better reliability compared to a simple portal vena cava anastomosis model. This model provides convenient conditions for the study of esophageal and gastric varices and endoscopic injection of sclerosing agents for the treatment of bleeding.