动物造模,动脉粥样硬化 zi-bio 2024-05-20 867

　　(1) The replication method involves injecting 10% monosodium glutamate subcutaneously into the back of newborn Wistar rats at a dose of 4g/kg body weight on the 1st, 3rd, 5th, 7th, and 9th day after birth, for a total of 5 injections. 30 days after injection of MSG, the aorta was taken for electron microscopy observation. Microvillous protrusions can be seen on the luminal surface of the endothelial cells in the aortic wall. The nuclei of the endothelial cells are somewhat twisted and irregular, and there is no thickening in the lower layer of the endothelium; 60 days after injection of MSG, the endothelial cells of the animal's blood vessel wall degenerated and shed, and the subendothelial layer significantly thickened. Many sized vacuoles and proliferative collagen fibers appeared, and macrophages and smooth muscle cells migrated into the subendothelial layer.

　　(2) Model characteristics This model is different from the food and drug induced As model. It destroys the arcuate nucleus of the hypothalamus by injecting monosodium glutamate, bypassing the process of vascular disease itself, and replicates the As model from the "central mechanism". Compared with similar models, such as long-term stimulation of the rabbit hypothalamus can cause hyperlipemia and As formation, destruction of the hypothalamus can aggravate coronary atherosclerosis, it has the advantages of simple method, accurate quantification, and reliable results.

　　(3) The histopathological characteristics of the animal model in comparative medicine after modeling include degeneration of endothelial cells in the aortic wall, swelling of cell nuclei, thickening and vacuolization of the subendothelial layer, and migration of smooth muscle cells to the subendothelial layer. These changes are consistent with the characteristic pathological changes of early onset of As in clinical practice; The substances closely related to the formation of As in the blood also undergo changes, with an increase in total cholesterol (TC), oxidized low-density lipoprotein (OX-LDL), and lipid peroxides (LPO), while a decrease in nitric oxide (NO). These changes are very similar to the changes induced by drug administration of As. This model can serve as a model for exploring the mechanism of central hypothalamic arcuate nucleus and As formation.