动物造模,肺水肿,药效评价 z-bio 2024-06-03 544

　　1. Animal modeling materials: Wistar rats, weighing (110 ± 10) g, with half male and half female; Drug: Liquid nitrogen tetroxide (N2O4), purity 99.5%; Equipment: 120L Poisoning Cabinet.

　　2. Modeling method: Animals in the modeling group were induced to inhale N2O4 [concentration of (352 ± 21) mg/m3] for 0.5 hours to replicate an acute pulmonary edema model. The method is to take 25 μ l of liquid N2O4 for the first time, inject it into a 120L toxicity cabinet, and place the model animal in the cabinet. Add 1 μ l of N2O4 to the cabinet every 5 minutes and inhale continuously for 30 minutes. Animals were euthanized 3 hours after poisoning, and only water was given without feeding before execution.

　　3. Modeling principle: Nitrogen tetroxide causes pulmonary edema in animals.

　　4. After modeling, compared with the control group, the lung coefficient of the modeling group was significantly increased (lung coefficient x 100, modeling group was 1.11 ± 0.2, control group was 0.59 ± 0.05), plasma MDA concentration was significantly increased [modeling group was (9.74 ± 0.6) nmol/ml, control group was (7.85 ± 0.4) nmol/ml], red blood cell SOD activity was significantly reduced [modeling group was (437.72 ± 61.5) ng/g hemoglobin, control group was (750.12 ± 100.27) ng/g hemoglobin], plasma ANP concentration was significantly increased [modeling group was (241.69 ± 30.8) pg/ml, control group was (157.53 ± 17.1) pg/ml, ANP in lung tissue was significantly reduced [modeling group was (7.14 ± 0.5) ng/g wet lung tissue, control group was (8.61 ± 0.72) ng/g wet lung tissue].