动物造模,药效评价,肺结核 z-bio 2024-06-27 484

　　1. Animal modeling materials: mice; Drug: Contains highly toxic human tuberculosis bacterium (H37RV).

　　2. Method of modeling: Animal models were made by injecting physiological saline into the tail vein, containing 1-5mg/ml of highly toxic human tuberculosis bacteria (H37RV), 0.2ml per animal.

　　3. Modeling principle: Mycobacterium tuberculosis infection leads to animal pulmonary tuberculosis.

　　4. Changes after modeling: Animals generally experience decreased appetite, reduced activity, weight loss, and fear of cold after vaccination. Death occurs 12-29 days after infection, with half of the survival time being 16 days.

　　Changes in cellular immune function: In the modeling group, Thi-1+(%) was 21.0 ± 3.77, L3T4+(%) was 9.8 ± 2.35, Lyt2+(%) was 10.0 ± 1.56, and L3T4+/Lyt2+was 0.98 ± 0.16; The control group had Thi-1+(%) of 40.0 ± 7.32, L3T4+(%) of 25.3 ± 5.56, Lyt2+(%) of 12.6 ± 2.12, and L3T4+/Lyt2+of 2.00 ± 0.17.