动物造模,药效评价,急性肝损伤 z-bio 2024-07-01 535

　　1. Animal modeling materials: BALB/c mice, weighing 20-30g, regardless of gender; Medications: Concanavalin A (ConA), PBS.

　　2. Modeling method: Dilute ConA with PBS and inject 20mg/kg into the tail vein.

　　3. The principle of modeling is that concanavalin A can activate the proliferation of CD8+or CD4+cells, increase the release of IFN - γ, and the latter promotes the infiltration of inflammatory cells into the hepatic portal vein area and activates the comprehensive response of M α function inducing factors, resulting in a cytotoxic effect on liver cells.

　　4. Changes after modeling. After 8 hours of injection, serum AST significantly increased; The pathological morphological changes show severe infiltration of neutrophils, lymphocytes, and monocytes in the liver lobules. Inflammatory lesions can also be seen in the portal and central venous areas, and electron microscopy shows diffuse hepatocyte necrosis.