动物造模,药效评价,急性肝损伤 z-bio 2024-07-01 414

　　1. Animal modeling materials: Kunming mice, weighing 18-22g, with half male and half female; Medication: Tetracycline tablets.

　　2. Modeling method: The tetracycline modeling group was prepared into a suspension at a dose of 2250mg/kg, and was orally administered once at a weight of 0.2ml/10g. After 18 hours, the model was formed; The blank control group was given an equal volume of physiological saline by gavage.

　　3. Modeling principle: Tetracycline antibiotics can cause serious liver damage. High drug dosage, intravenous injection, and renal dysfunction can cause excessive blood drug concentration, leading to fatal acute hepatic steatosis.

　　4. Changes after modeling After 18 hours of modeling, ALT and AST increased significantly (about three times that of the blank group); Significant steatosis and edema were observed in all liver cells, and the hepatic sinuses were significantly narrowed or disappeared. The pathological changes of liver cells were particularly severe after 54 and 72 hours. The entire field of view is filled with fatty liver cells, and the liver sinuses have completely disappeared.

　　Electron microscopy observation showed that the mitochondria in the liver cells of the blank group mice were abundant, with most of the mitochondrial cristae densely arranged and even intercristal cavities; Rich endoplasmic reticulum; The Golgi apparatus is arranged in multiple layers; The nucleus often contains more chromatin and less heterochromatin; There is no abnormal material structure in the cytoplasm. The liver cells of the model group mice were occupied by a large amount of lipid droplets; Mitochondria significantly decreased; Most of the mitochondrial cristae have disappeared, with only a few fragments remaining; The endoplasmic reticulum and Golgi apparatus are also difficult to find and are basically in a state of fragmented edema; The nucleus often has an increase in chromatin, while the heterochromatin is significantly reduced.