动物造模,药效评价 z-bio 2024-07-01 271

　　(1) Reproduction method: Adult mice, good at calling ferocious cats. When raising mice to the age of 75 days, they were mated in a ratio of 2:1 male and female. On the evening of the 4th day, male mice were taken out, and on the 5th day, the experimental mice were intimidated. The pregnant mice were placed directly under a cat cage, which was only separated by a net from the cage. The cat's paws could reach the female mice, and they were intimidated for 2 hours in the morning and 2 hours in the afternoon every day, for a total of 14 days, until 1 day before the delivery of the pregnant mice. Feed 1-2 male mice or non experimental mice to cats during each intimidation to increase the atmosphere of intimidation. On the 19th day after mating, the mother mice began to give birth one after another. Some newborn mice were euthanized and their testicles were taken for relevant examinations. The remaining animals were raised for 75 days and euthanized, and their testicles were taken for relevant examinations.

　　(2) Model characteristics: HE staining of the right testicle of offspring male mice revealed significant edema in the stroma of spermatogenic cells, with some having loose and swollen cytoplasm and some accompanied by the formation of vacuoles of varying sizes in the cytoplasm. Sperm production decreased in the lumen of the seminiferous tubules, and the number of layers of spermatogenic cells showed an upward trend. The sample showed a significant increase in spermatogenic cells within 880 μ m3. The offspring mice were raised for 75 days and euthanized. The ratio of testicles to body weight of male mice born to threatened pregnant mice showed no significant abnormalities. When the left testicle was refrigerated for 15 days, it was found that there were differences in dryness, wetness, and color compared to normal mice, with a significant increase in coefficient. After 60 days of freezing, the testes of offspring mice were soaked in a 10% formaldehyde solution for 31 days. After slicing, HE staining was used to observe under the light microscope. The number of layers of spermatogenic cells in the seminiferous tubules was 4-5, and the arrangement and distribution of spermatogenic cells were uneven. There was obvious edema in the interstitial space of spermatogenic cells, and most of the cytoplasm of spermatogenic cells had vacuolar degeneration, with half of the perimeter unclear and some showing obvious decay. A small amount of scattered macrophages and a few forming multinucleated giant cells were also observed in the seminiferous tubules. Sperm production in the lumen was significantly reduced and unevenly distributed.

　　(3) Comparative medicine is based on the traditional Chinese medicine theory that newborn babies are weak and thin, which belongs to fetal timidity and insufficient kidney essence. Animals with significant weight loss within 3 days of birth are screened, or the traditional Chinese medicine theory of fear of damaging the kidney is used to intimidate pregnant mice and cause congenital kidney deficiency in offspring. Fetal timidity results in immature intrauterine development and insufficient innate endowments. Manifested as delayed growth and development, low physiological function, and characterized by spleen and kidney deficiency, with kidney deficiency being particularly prominent. Research has found that fetal timidity models exhibit endocrine disorders such as pituitary, thyroid, and adrenal glands, while offspring with congenital kidney deficiency caused by threatening pregnant mice exhibit behavioral abnormalities.