动物造模,药效评价,慢性肝损伤 z-bio 2024-07-01 242

　　1. Animal modeling materials: Rats, weighing 200-250g, regardless of gender; Medications: sucrose, alcohol.

　　2. Starting from 3 days of modeling, rats were given a 2% sucrose solution for free consumption, and then 5% alcohol was added to the sucrose solution. The alcohol concentration was increased by 5% to 15% every 4 days, and then changed to a weekly increase of 5% until the final alcohol concentration reached 40% (accounting for 39% of the total daily calories). At the same time, low fat (7% calories), high protein (13% calories), and solid feed containing choline and carbohydrates (41% calories) were fed for 16-29 weeks.

　　3. Principle of modeling: Alcohol causes chronic liver damage.

　　4. Changes after modeling: Animal liver cells in the modeling group showed steatosis, inflammatory liver cell necrosis, and intralobular fibrosis. Electron microscopy observation showed abnormal mitochondria and significantly proliferative smooth endoplasmic reticulum.