动物造模,药效评价,慢性肝损伤 z-bio 2024-07-01 301

　　1. Animal modeling materials: Kunming mice, weighing 25-30g, regardless of gender; Medications: BCG vaccine, Escherichia coli lipopolysaccharide (LPS).

　　2. Method of modeling: Prepare a BCG vaccine solution with physiological saline to contain 10 times the power of live bacteria per milliliter, and inject 0.2ml (2.5mg) of BCG vaccine solution through the tail vein. 10 days later, 0.2ml (10 μ g) of LPS physiological saline solution was injected daily through the tail vein. Blood collection and liver tissue testing after 16 hours.

　　3. Principle of modeling: Pre injection of BCG vaccine into mice can induce the aggregation of multinucleated neutrophils or macrophages in the liver, followed by low-dose injection of E. coli lipopolysaccharide, which can stimulate the release of soluble factors with toxic effects on liver cells and cause immune liver injury.

　　4. Changes after modeling: The serum ALT and AST values increased after modeling (reaching a peak 12 hours after LPS injection), and the pathological changes in the liver were mainly granulomatous inflammatory infiltration.