动物造模,药效评价,银屑病 z-bio 2024-07-01 316

　　1. Application of propranolol to create a guinea pig ear psoriasis model. This model is commonly used in China. Huang et al. applied 5% propranolol to the ear of guinea pigs (350-400g) and 0.3g of the drug (1cm2) twice a day for 2 consecutive weeks. At the same time, 1% dinitrochlorobenzene (DNCB) and 1% croton oil castor oil were used externally as the control group. The results confirmed that psoriasis like skin lesions appeared in the ears of guinea pigs in the propranolol group, and this skin lesion was caused by the unique pharmacological effects of propranolol, rather than irritating dermatitis.

　　Jensen et al. selected hairless rats of no more than 3 weeks old, regardless of gender, for the psoriasis model in hairless rats with essential fatty acid deficiency. At the beginning of the experiment, the average weight of the rats was 47.5g. Each rat is raised separately, eating freely and drinking water. It is given 12 hours of light per day, with a temperature of 22-26 ℃ and a humidity of about 30%. The food used for feeding lacks essential fatty acid deficiency (EFAD), which is formulated with 20% mass fraction of edible casein (containing 0.01% linoleic acid), 70% sucrose, 5% cellulose, and 5% vitamins and inorganic salts. The construction of the model was evaluated based on indicators such as animal weight, water consumption, transdermal water loss, clinical skin manifestations, histopathology of the epidermis, and fatty acid content in serum and skin. The results showed that the model could be successfully established within 8 weeks.

　　In addition, ultraviolet radiation can stimulate the skin cells of nude mice to accelerate renewal, resulting in excessive keratinization and simulating psoriasis.