动物造模,药效评价,脑卒中后遗症 z-bio 2024-07-01 355

　　1. Animal modeling materials: 12 week old male Wistar rats, weighing 250-300g; Medication: Anesthetics; Instruments: rat stereotactic device, miniature electrocoagulation knife, electric pulse random stimulation device.

　　2. Method of modeling: After anesthesia in rats, all openings were made in the middle of the neck. The left common carotid artery was ligated first, and then the right common carotid artery was clamped and released for 1 hour. The animal was fixed on the rat's stereotactic device, and all openings were made above the line connecting the right ear edge and eyelids. The skull was exposed, and a bone window with a diameter of about 2mm was drilled. The middle cerebral artery was closed with a miniature electrocoagulation knife. After 24 hours of surgery, a rat random electric pulse stimulator was used with stimulation intensity of 50-80V, 0.5-25Hz, 0.1-1.1ms. The model was established 20 days later.

　　3. Principle of modeling: The electrocoagulation method was used to create a rat model of middle cerebral artery occlusion (MCAO).

　　4. After modeling, compared with the normal group, all groups of rats showed symptoms of hemiplegia, characterized by dull hair, lethargy, drowsiness, reduced food and water intake, left upper limb adduction, weakness, body tilting to one side when climbing the rat cage, lower limb abduction, difficulty in adduction, limping when walking or jumping, inability to roll up normally when lifting the tail, and constant shaking of the body. Male rats had left testicular atrophy, manifested as different sizes of testicles on both sides.

　　Comparison of neurological deficit scores: 20 days after surgery, the model group's scores were significantly lower than those of the normal group; 35 days after surgery, the score of the model group was significantly lower than that of the normal group, but there was also a downward trend.

　　Pathological changes in brain tissue: Under the light microscope, it can be seen that the neuronal cells in the infarcted area of the model group rats shrink, the nuclei partially dissolve or disappear, the neuronal cell bodies at the edge of the infarcted area are enlarged, the cell boundaries are blurred, glial cells are rare, and the tissue distribution is reticular.

　　Ultrastructural changes in brain tissue: Under electron microscopy, significant edema and vacuolar changes were observed in both neurons and glial cells of the model group rats. The neuronal cell membrane was swollen and ruptured, the nuclear membrane was wrinkled and uneven, the nucleolus disappeared, the mitochondria were swollen, the mitochondrial membrane was ruptured, the rough endoplasmic reticulum expanded, and myelin sheaths were often separated and damaged. Glial edema and vacuolar changes were observed around the capillaries, and red blood cells clustered in the capillary wall.

　　The levels of ATP and ADP in the brain tissue of the model group rats were significantly lower than those of the normal group. The levels of NE, dopamine, and serotonin in the brain tissue of the model group rats were significantly lower than those of the normal group.