动物造模,药效评价 z-bio 2024-07-01 265

　　(1) Method of modeling: 36 New Zealand white rabbits were used, with 18 rabbits in the experimental group and 18 rabbits in the control group. On the 1st to 5th day of the experiment, daily intravenous injection of 440mg/m2 of cytarabine was administered to the ear margin, and immunosuppressive status was maintained every other day after 6 days. Starting from the 4th day, all rabbits were given intravenous injections of vancomycin 15mg/kg and ceftazidine 150mg/kg, once a day; Gentamicin 5mg/kg intravenous injection, once every other day to prevent bacterial infection. On the 6th day, a 0.2ml suspension of Candida albicans (with a concentration of 5 × 10 to the 8th power/ml) was injected through percutaneous tracheal puncture, while the control group was injected with an equal amount of physiological saline. On the 1st to 6th day after 24 hours of vaccination, animals were euthanized in the designed order, and lung tissue was taken to observe pathological changes; Ligate the left and right main bronchi at the distal end of the protuberance, and repeatedly inject and aspirate 1ml of sterile physiological saline twice. Rinse the rinse solution into sterile test tubes for later use.

　　(2) Result: The granulocyte count in the venous blood of the rabbit ear margin significantly decreased after injection of immunosuppressants; The experimental group of 16 rabbits (16/18) showed varying degrees of inflammatory changes in the lungs, and spores and hyphae were found in the affected areas. Thickening of the bronchial wall, infiltration and growth of a large number of fungal spores, and proliferation of lymphocytes on the tube wall. The surrounding alveolar wall thickens and there is a small amount of protein extract in the cavity. The local alveolar structure disappears and appears uniformly filled with necrotic material, showing a large amount of hyphae and spores.