动物造模,药效评价,创伤性脑水肿 z-bio 2024-07-01 270

　　1. Animal modeling materials: Healthy adult male SD rats, weighing 210-300g; Medication: pentobarbital, 10% glucose physiological saline.

　　2. Method of modeling: The animal in the modeling group underwent surgery to expose the left parietal bone. A bone hole with a diameter of 5.0mm was drilled along the midline of the sagittal suture and 1.5mm behind the coronal suture, exposing the dura mater. The lower end of the cone was impacted with a weight of 40g and a height of 25cm, with a diameter of 4.5mm and a height of 2.5mm, resulting in focal severe brain contusion.

　　3. The principle of modeling is that trauma leads to brain edema in animals.

　　4. Changes after modeling: Compared with the sham surgery group, the model group showed an increase in brain tissue water content. On day 1, 3, 5, and 7, the model group had brain water content of (81.68 ± 0.23)%, (82.55 ± 0.20)%, (81.95 ± 0.36)%, and (79.73 ± 0.46)%, respectively. The sham surgery group had brain water content of (77.96 ± 0.39)%, (78.17 ± 0.31)%, (77.95 ± 0.28)%, and (78.05 ± 0.45)%, with significant differences. The highest was observed on day 3 after injury and the lowest was observed on day 7.

　　Compared with the sham surgery group, the sodium potassium ATPase content in the model group decreased. On days 1, 3, 5, and 7, the sodium potassium ATPase content in the model group [μ mol Pi/(mg · prot · h)] was 0.5245 ± 0.0204, 0.4064 ± 0.0197, 0.5061 ± 0.0144, and 0.6098 ± 0.0156, respectively. The sodium potassium ATPase content in the sham surgery group was 0.8485 ± 0.0152, 0.8554 ± 0.0181, 0.8438 ± 0.0166, and 0.8522 ± 0.0169, with significant differences.