动物造模,药效评价 z-bio 2024-07-01 371

　　1. Animal modeling material: Rabbits; Medications: Anesthetics, typhoid lipopolysaccharide, 2.5% Evans blue physiological saline; Instruments: Brain pressure gauge, multi-channel physiological instrument.

　　2. Method of modeling: After animal anesthesia, the common carotid artery is surgically separated and a right ventricular catheter is inserted. A brain pressure gauge is connected to measure ventricular pressure, and an amplifier can also be connected to monitor intracranial pressure (ICP) using a multi-channel physiological instrument. Inject 2.5% Evans blue physiological saline 1ml/kg into the ear vein, and after ICP remains constant, inject typhoid lipopolysaccharide 4.008mg/kg from the right common carotid artery.

　　3. The principle of modeling is that typhoid lipopolysaccharides cause brain edema in animals.

　　4. Changes after modeling: Model animals exhibit symptoms of cerebral edema, and generally, animals can survive for more than 6 hours.