动物造模,药效评价 z-bio 2024-07-01 427

　　1. Animal modeling materials: SD rats, weighing 142-180g; Medication: Sucrose.

　　2. Model making method control group, randomly eating standard feed and drinking tap water; Sucrose group, eating standard feed freely, drinking 10% sucrose and tap water (w/v,%). The experiment lasted for 6.5 weeks.

　　3. The principle of modeling is that high sugar intake can cause insulin resistance and hyperinsulinemia in SD rats, leading to an increase in blood pressure. The possible mechanism of the latter is: ① insulin induced sodium water retention; ② Stimulating sympathetic nervous system activity increases; ③ The activity of Na+and Ca2+pumps on the cell membrane decreases, leading to an increase in intracellular calcium; ④ Insulin mediated changes in vascular smooth muscle tension.

　　4. Changes after modeling: The systolic blood pressure (SBP) of the model rats was (17.04 ± 0.41) p/kPa before modeling. After 3-6 weeks of modeling, it was (19.08 ± 0.98) p/kPa.