动物造模,药效评价,肾动脉狭窄性高血压 z-bio 2024-07-01 402

　　1. Animal modeling materials: Dogs or rabbits, regardless of gender; Medications: anesthetics, antibiotics.

　　2. Method of animal modeling: After anesthesia, the animal is placed in an abdominal position, starting from 1.5-2cm beside the spine, with the right side along the rib edge and the left side making a 4cm skin incision about two fingers wide at the rib edge. The subcutaneous tissue of the lumbar and dorsal fascia is separated, the inner oblique muscle fascia is cut open, the long dorsal muscle is pushed open, the kidney is exposed, and a segment of the renal artery is carefully bluntly separated. A silver clip or ring of a certain diameter is used to cover the renal artery to cause renal artery stenosis. If one side of the renal artery is narrow, the other side of the kidney is removed after an interval of 10-12 days. After surgery, inject antibiotics to prevent infection.

　　3. The modeling principle is that one side of the kidney is resected, and the other side of the kidney is ischemic due to narrowing the renal artery on that side with a silver clip, so the increase in blood pressure is mainly due to sodium and water retention, activation of renin-angiotensin system, and increased sympathetic nerve activity.

　　4. Changes after modeling: A few days after surgery, the animal's blood pressure begins to rise, and after 1-3 months, it reaches its peak and can be maintained for a long time.