动物造模,药效评价,乳腺癌 z-bio 2024-07-08 613

　　The induced tumor model is the use of chemical, biological carcinogens or radiation to induce mouse tumors under certain conditions, mainly through chemical induction of tumors. The commonly used inducers are methylnitrosourea or DMBA, which are used to induce breast cancer in experimental animals by gavage, local application, subcutaneous injection or intravenous injection. After BALB/c mice were induced by breast tumor virus, the incidence rate of breast cancer was higher.

　　【 Modeling Method 】 Using acetic acid with pH 6.0 as the solvent, prepare a MNU solution with a concentration of 5mg/ml, and use it within 30 minutes. Wistar rats, female, 21 days old, weighing approximately 40-50g, were administered a single intraperitoneal injection of MNU at a dose of 50mg/kg. It takes 5 weeks, and after 3 weeks of injection (42 days old), the mammary glands of rats were touched daily, with a tumor formation rate of 83.3% by the 5th week. Wistar rats were perfused with low-dose DMBA for 3 months, and the animal model of breast cancer lesions could also be established.

　　【 Model characteristics 】 MNU induced malignant tumors have high invasiveness and are often estrogen dependent. The MNU induced rat model experienced the pathological stage from inverted ductal papilloma to breast ductal carcinoma to invasive ductal carcinoma, which was completely consistent with the histological process of the occurrence and development of breast cancer.

　　The evaluation and application of the induced tumor model is easy to operate, with stable target organs and carcinogens, high induction rate of cancer, long doubling time of tumor cells, low proliferation rate, similar to the dynamic characteristics of human tumor cells. Therefore, the chemically induced animal model can be used for the etiology research, preventive research and comprehensive chemotherapy effect evaluation of breast cancer. However, due to the long establishment period of induction model and the large morphological difference of tumor cells, it is not commonly used in animal models for screening anti breast cancer drugs.