动物造模,药效评价,阿司匹林脾不统血证 z-bio 2024-07-17 351

　　(1) Method of replication: Adult rats were given a dose of 50% medical ethanol 1ml/100g body weight by gavage on the first day, a dose of 1ml/100g body weight of Baoning vinegar by gavage from the 2nd to the 11th day, and a dose of 1% aspirin 1ml/100g body weight by gavage from the 12th to the 25th day. At the end of the experiment, red blood cell, white blood cell, platelet counts, red blood cell C3b receptor rosette rate, and red blood cell C3b receptor immune complex rosette rate were measured. The animals were euthanized by decapitation, and thymus and spleen were taken for histological examination.

　　(2) Model features: animals with vertical hair, diarrhea, decreased appetite, weight loss, decreased body temperature, decreased spontaneous activity, positive fecal occult blood test, and prolonged coagulation time; Autopsy shows punctate or patchy bleeding in the stomach and duodenum of animals; Under light microscopy, it can be seen that the thymic cortex has significantly become thinner, the number of lymphocytes in the cortex has decreased, the PAS positive reticular epithelial cells in the thymic medulla have significantly decreased, and the T and B cell regions in the spleen have significantly shrunk; The rates of white blood cells, red blood cells, platelets, RBC-C3b receptor garlands, and RBC-IC garlands decreased.

　　(3) Comparative medical wine is a dry and strong product, vinegar is a sour tasting agent, and wine vinegar can cause spleen deficiency. Spleen deficiency is the basis of spleen failure to regulate blood flow, and there must be triggering factors for spleen deficiency bleeding. Among many causes of spleen deficiency bleeding, aspirin is used in small amounts (this amount alone is not easy to bleed), the model is stable, and the repetition rate is high.