动物造模,药效评价,营养不良血虚证 z-bio 2024-07-17 327

　　(1) The replication method involves drawing blood from the central ear artery and femoral artery of adult rabbits multiple times in a row, once every other day, with no more than 10% of their total blood volume each time, averaging 8-10ml, for 2 weeks. During this period, food intake is limited to 1/2 of the original amount, and water intake is not restricted. Detect changes in body weight, body temperature, appetite, fur, urine and feces, mental status, conjunctival microcirculation, blood routine, atrial natriuretic peptide, hemodynamics, etc. of rabbits.

　　(2) After 2 weeks of bloodletting and starvation, the animals showed withered fur, pale eyelids and lips, curled up with little movement, emaciation, significant decrease in hemoglobin, red blood cells, hematocrit, and whole blood viscosity, a tendency to decrease in serum and plasma viscosity, and a significant increase in plasma atrial natriuretic peptide. Microcirculation testing of the conjunctiva shows that its color is mostly light red, and the number of intersection points is significantly reduced, indicating that blood flow perfusion decreases and the opening of the capillary network decreases in cases of blood deficiency; The diameter of microvessels decreased in all cases, but the ratio did not change significantly; Micro blood flow velocity increases with more granular flow, and there are also visible broken lines; There was no significant change in the aggregation of red blood cells; The microcirculation contour of the conjunctiva is mostly blurred, with poor clarity and slight exudation. Old pigmentation can be seen in the tube loops.

　　(3) Comparative medicine bloodletting can cause blood loss, while 'inability to quickly generate visible blood' can lead to blood deficiency; Hunger causes a lack of qi in the middle burner, and if there is no juice available, the biochemical deficiency of blood will also lead to blood deficiency. This model uses a combination of bloodletting and hunger factors to create a model, reflecting the characteristics of syndrome differentiation and etiology. Symptoms, weight, microcirculation, hemodynamics and other indicators also reflect the overall concept of traditional Chinese medicine, and are simple and easy to implement. Its modeling time limit and rehabilitation cycle are relatively long, similar to clinical blood deficiency syndrome.