动物造模,药效评价,缺血性贫血 z-bio 2024-07-18 407

　　Hemolytic anemia is a common type of anemia, which refers to a type of anemia caused by the shortening and increasing destruction of red blood cells due to certain reasons, exceeding the compensatory capacity of the bone marrow. It is a common hematopoietic system disease.

　　[Model making mechanism] Animals are injected with a certain amount of acetylphenylhydrazine (APH), which is a strong oxidant that can specifically cause slow and progressive oxidative damage to red blood cells, especially by interfering with glucose-6-phosphate dehydrogenase in red blood cells, promoting hemoglobin denaturation and forming Hay's bodies. It can also directly damage the membrane proteins and lipids of red blood cells, causing membrane dissolution and rupture, red blood cell disintegration, and resulting in hemolytic anemia.

　　[Method of Modeling]

　　On days 1, 4, and 7, mice were subcutaneously injected with APH physiological saline solution at doses of 0.2g/kg, 0.1g/kg, and 0.2g/kg. On day 9, the model was successfully established, and other administration methods can also create this model.

　　On the 1st and 4th days, rats were subcutaneously injected with 0.16g/kg and 0.08g/kg of APH physiological saline solution, and successful modeling was achieved on the 8th day; Or both doses were 0.2g/kg, and the modeling was successful on the 10th day.

　　3. Rabbits can establish a hemolytic anemia model by subcutaneous or intramuscular injection of 2% APH physiological saline solution 2-3 times at a dose of 0.1g/kg.

　　Observation indicators: General growth status observation, including general behavior, hair, bleeding points, and mortality status; Measurement of peripheral blood routine parameters, such as red blood cell count, white blood cell count, platelet count, and hemoglobin content; Changes in blood cell chemical indicators, such as alkaline phosphatase, acid phosphatase, triphosphate adenylate kinase, succinate dehydrogenase, glucose-6-phosphatase, reticulocyte count, Hay's body, bone marrow examination, and other indicators of neutrophils.

　　【 Model Characteristics 】 After injection of APH, the experimental group animals gradually showed reduced activity, dull and dull hair, decreased feeding, weight loss, fatigue and weakness, drowsiness, pale skin, and other phenomena. Progressive decline in peripheral blood hemoglobin and red blood cells; The total number of reticulocytes, Haematosomes, and white blood cells significantly increased. Neutrophil alkaline phosphatase, acid phosphatase, triphosphate adenylate kinase, succinate dehydrogenase, glucose-6-phosphatase, and bone marrow all exhibit varying degrees of abnormal changes.

　　Model Evaluation and Application: An animal model of hemolytic anemia that meets clinical characteristics can be created by injecting the strong oxidant APH. The dosage of APH can be explored through preliminary experiments, and the above dosage can be used as a reference. This method has a short modeling cycle and is easy to operate. The symptoms, peripheral blood count, and biochemical changes of blood cells in the model animals are similar to those in human hemolytic anemia, providing a simple model for studying experimental hemolytic anemia.