动物造模,药效评价,T细胞移植银屑病 z-bio 2024-07-18 470

　　Although the specific pathogenesis of psoriasis is not fully understood, the role of T lymphocytes in it is increasingly supported by evidence.

　　【 Modeling mechanism 】 Severe combined immunodeficiency mice (SCID mice) lack functional lymphocytes and are often used for immune cell differentiation and functional research. It is a commonly used animal model in research fields such as heterologous immune function reconstruction, human autoimmune diseases, immunodeficiency diseases, infections, and tumors. Transplanting CD4+/CD45RHiT lymphocytes into SCID mice can induce a psoriasis model.

　　【 Model Characteristics 】 The incidence of psoriasis like symptoms in successfully transplanted mice is 100% within 4-8 weeks. However, SCID mice transplanted with CI4+/CD4SRBloT lymphocytes did not exhibit psoriasis like appearance, and after transplantation of CD4+/CD45RBhi and CD4+/CD45RBloT lymphocytes, the symptoms of the mice were alleviated. Narrow spectrum ultraviolet radiation and cyclosporine A both have therapeutic effects on this model.

　　The success of the T cell transplantation model demonstrates the important role of dysfunction of regulatory T cell subsets in the pathogenesis of psoriasis. Even in the absence of primary epidermal cell abnormalities, psoriasis can still be induced. The construction of this model has propelled psoriasis research from the stage of "single factor" to the stage of "holistic" research. In addition, this model can also be used for screening drugs for the treatment of psoriasis.