动物造模,药效评价,异种动物原位移植骨肿瘤 z-bio 2024-07-18 330

　　[Modeling mechanism] Usually, tumor blocks or cells are implanted subcutaneously in nude mice, and the tumor continues to grow, which can simulate the process of human malignant tumor formation. The tumor formation rate is high, the tumor formation time is uniform, the tumor size and weight are easy to measure, and it is easy to conduct basic and clinical research. However, the growth, invasion, and metastasis behavior of tumor transplants are not only related to the biological characteristics of the tumor itself, but also closely related to the transplant site of the tumor. Subcutaneous inoculation causes tumor cells to detach from the microenvironment of their originating tissue and organ. In experiments, certain biological behaviors of transplanted tumors often differ from those of malignant tumors in the original patient, which is considered ectopic transplantation; The corresponding in situ transplantation is a new type of tumor transplantation method that is increasingly recognized by people. It involves inoculating human tumors into the host organ tissue corresponding to the primary tumor site, creating a microenvironment similar to that of human tumors and providing various endogenous growth factors that promote tumor growth.

　　[Method of Modeling]

　　1. Logarithmically growing UMR-106 cells were taken using the cell suspension method. After trypsin digestion, the cells were washed twice with PBS to remove residual serum. Finally, the cell volume and concentration were adjusted using serum-free culture medium RPML l640. Female BALB/c-nu/nu nude mice were anesthetized with Uratan at a dose of 600 μ l/g. Prior to inoculation, the local skin was routinely disinfected, and the lateral femur was exposed under a surgical microscope. A 0.15mm × 0.15mm sized cortical bone was lifted up, and the cell suspension was slowly injected into the medullary cavity. The bone flap was reset, sealed with bone wax, and the fascia and skin were sutured. The entire process was performed in a sterile purification room.

　　2. The tissue block method was used to obtain logarithmic growth phase UMR-106 cells, adjust the cell concentration, and inoculate 2 × 1000000 cells per mouse subcutaneously on the back of 4-week female nude mice. After 4 weeks, the transplanted tumor increased to 2.0cm × 2.0cm × 1.5cm. Take non necrotic tumor tissue and place it in PRMI 1640 medium. Cut it into tumor tissue blocks with a diameter of approximately 0.3mm using ophthalmic scissors for future use. Take 6-week-old female nude mice and open the lateral femur using the same method as above. Transfer the tumor tissue block that has been trimmed to a uniform volume into the bone marrow cavity, reposition the bone flap, and suture it. Usually, the obvious growth trend only appears in the third week of film formation.

　　[Model features] Starting from the second week after implantation of tumor tissue blocks or inoculation of cell suspension, the bone area begins to enlarge, showing an expansive increase, squeezing surrounding tissues, presenting a purple red appearance of skin and swollen veins. After 3-4 weeks of implantation, X-ray images showed bone destruction and surrounding soft tissue infiltration, and pathological fractures appeared 6 weeks later. Under light microscopy, transplanted tumor cells vary in size and shape, with large and deeply stained nuclei, inverted nuclear cytoplasmic ratios, and nest like distribution.

　　The evaluation and application of the model can well simulate the original growth microenvironment of osteosarcoma, with advantages such as less tumor ischemic necrosis, high lung metastasis rate, and dense metastatic lesions. Mainly used for studying the biological characteristics of tumor cell growth and invasion, for localization imaging diagnosis of osteosarcoma, and limb salvage treatment; Used for research on targeted therapy carriers and chemotherapy drug sensitivity screening.