动物造模,药效评价,反流性胃炎 z-bio 2024-07-22 311

　　1. Modeling material animals: Male rats weighing 200-250g; Medications: Pentobarbital, physiological saline, iodine.

　　2. Modeling method

　　(1) Model rats were anesthetized by intraperitoneal injection of 30mg/kg pentobarbital, disinfected with iodine, and opened to expose the stomach and upper jejunum. The anterior stomach was fixed to the jejunum 5-6cm away from the pylorus, leaving a 7-8mm incision in the middle. First, a 6-7mm incision was made in the anterior stomach, and the stomach cavity was carefully washed clean. Be careful not to flush the stomach contents into the abdominal cavity. Then, a small incision of the same size was made in the small intestine. The inner side of the stomach and small intestine incision were intermittently sutured (using No. 0 silk thread), and the outer side was sutured. The incision was closed, and the anastomosis was completed. The abdominal cavity was washed twice with disinfectant saline and dried with clean gauze. The abdominal wall muscle layer and skin were sutured layer by layer, and the abdominal cavity was closed. Fasting for 1 day after surgery and modeling for 3 months.

　　(2) Male SD rats were anesthetized with pentobarbital (30mg/kg, intraperitoneal injection). Cut off the abdominal hair, disinfect the skin, open the upper abdomen, expose the stomach and upper jejunum, and fix the anterior stomach with the jejunum 5-6cm away from the pylorus. Leave 7-8mm in the middle for all openings. Firstly, make a 6-7mm incision in the anterior stomach, carefully rinse the stomach cavity with physiological saline, and then make an equally large incision in the small intestine. First, suture the inside of the incision between the stomach and the small intestine, then suture the outside to seal the incision. Rinse the abdominal cavity twice with physiological saline, dry it with disinfectant gauze, and inject 2-3 drops of gentamicin diluent. Suture the muscle layer and skin in layers, close the abdominal cavity and add 2-3 drops of gentamicin dilution solution, and fast for 24 hours.

　　3. The principle of modeling surgery leads to animal reflux gastritis.

　　After 3 months of modeling, pathological sections showed significant infiltration of inflammatory cells, glandular hyperplasia, and intestinal metaplasia; Visible to the naked eye is congestion and blood stasis of the gastric mucosa, arranged in a pebble like pattern.

　　5. Pathological changes after modeling mainly occur in the mucosa around the gastrointestinal anastomosis, including infiltration of inflammatory cells, atrophy of gastric glands, intestinal metaplasia of gastric mucosa, and width of gastric pits. Inflammation is mainly characterized by the number of lymphocytes, mostly occurring in the submucosal lamina propria, and some specimens have infiltrated into the mucosal muscle layer and submucosal layer.