动物造模,药效评价,诱发性银屑病 z-bio 2024-07-22 504

　　1. Propranolol application induced ear psoriasis model in guinea pigs. This model is commonly used in China. Huang et al. applied 5% propranolol to the ear of guinea pigs (350-400g) and the ear of guinea pigs (0.3g per 1cm2 of drug) twice a day for two consecutive weeks. At the same time, 1% dinitrochlorobenzene (DNCB) and 1% croton oil castor oil were used topically as the control group. The results confirmed the presence of psoriasis like skin lesions on the ears of guinea pigs in the propranolol group, which were caused by the unique pharmacological effects of propranolol rather than irritant dermatitis.

　　2. Jensen et al. selected hairless rats no older than 3 weeks old, regardless of gender, as the psoriasis model of hairless rats lacking essential fatty acids. At the beginning of the experiment, the average weight of the rats was 47.5g. Each rat is raised separately, with free access to food and water. It is given 12 hours of light exposure daily at a temperature of 22-26 ℃ and a humidity of approximately 30%. The food used for feeding lacks essential fatty acid deficiency (EFA), and its formula consists of 20% mass fraction of edible casein (containing 0.01% linoleic acid), 70% sucrose, 5% cellulose, and 5% vitamins and inorganic salts. The model construction was evaluated based on animal weight, water consumption, transdermal water loss, clinical skin manifestations, histopathology of the epidermis, and fatty acid content in serum and skin. The results showed that the model could be successfully established within 8 weeks.

　　In addition, ultraviolet irradiation can stimulate the accelerated renewal of epidermal cells in nude mice, leading to hyperkeratosis and simulating psoriasis.