动物造模,药效评价,肺氧化损伤 z-bio 2024-07-23 304

　　1. Modeling material animals: 3-month-old male SD rats; Equipment: O3 generator, dynamic poisoning box.

　　2. Modeling method: Except for the blank control group, all other groups of rats were placed in a dynamic poisoning box connected to an O3 generator at a concentration of 5mg/m3 for 8 hours a day, fed with regular drinking water for 3 weeks.

　　3. Modeling principle: Ozone is a strong oxidant that is not a free radical itself. However, when inhaled into the body, it can quickly convert into highly active free radicals - superoxide anion free radicals. Excessive free radicals exceed the body's ability to eliminate them, disrupting the balance between production and elimination and causing damage to the body. This is mainly achieved through the peroxidation of unsaturated fatty acids in biological membranes, leading to membrane structure and functional impairment, as well as reduced physiological functions.

　　4. Compared with the blank control group rats [SOD was (418.7 ± 74.01) U/ml, MDA was (5.76 ± 1.53) nmol/ml, GSH Px was (192.55 ± 32.75) U/ml], the serum SOD (278.68 ± 53.5) U/ml and GSH Px (115.26 ± 20.17) U/ml activities in the O3 injury model group rats were significantly reduced, while the MDA (10.21 ± 2.86) nmol/ml content was significantly increased.