动物造模,药效评价,发绀型心脏病 z-bio 2024-07-23 255

　　1. Modeling material: New Zealand white rabbit, male, 5-6 months old, weighing 1.5-2.0kg; Drug: Sodium thiopental.

　　2. The modeling method involves anesthetizing animals with intravenous injection of 30mg/kg thiopental sodium at the ear border, followed by intravenous injection of 3-5mg/kg every 30 minutes for maintenance. After shaving the skin of the front chest and disinfecting it, a midline incision is made to cut open the skin, subcutaneous tissue, and muscle layer. The sternum is horizontally transected at the 7th intercostal space, and the sternum is cut upwards along the midline. A stretcher is used to open the sternum, and a heart bag is cut to expose the heart. After freeing the main pulmonary artery, the root of the left atrial appendage was ligated with a double knot of No. 4 silk thread to block blood flow. A 4-5mm incision was made in the middle and posterior part of the right edge of the left atrial appendage, and 1% heparin was injected into the auricle. The left wall of the main pulmonary artery was clamped with a self-made sidewall clamp, and a 4-5mm incision was made in the corresponding area of the left atrial appendage. The pulmonary artery was anastomosed to the left atrial appendage side by side with 8-0 non-destructive nylon suture thread. The posterior wall was sutured continuously, and then the anterior wall was sutured intermittently. Before tying the suture, exhaust the air. First, loosen the ligature at the base of the left atrial appendage, and then slowly loosen the sidewall clamp on the pulmonary artery. The control group was the same as the modeling group except for not performing anastomosis between the main pulmonary artery and the left atrial appendage.

　　3. Modeling principle: Based on the etiology of cyanotic heart disease, an animal model of cyanotic heart disease is established by anastomosing the pulmonary artery with the left atrial appendage.

　　4. After modeling, there is a general change. When the intraoperative anastomosis is completed, the color of the left atrial appendage can be seen to have significantly darkened in the modeling group, while there is no change in the control group. Four weeks after surgery, after animal sacrifice, mild ventricular dilation was observed in the cyanotic group, particularly in the right ventricle. The anastomotic site was unobstructed with a diameter of 3-4mm and smooth.

　　5. Biochemical and pathological changes after modeling. Postoperative arterial blood gas analysis and blood routine measurement results showed that in the first week after surgery, the arterial oxygen pressure (PO2) and arterial oxygen saturation (SaO2) in the modeling group were significantly reduced compared with the control group, with significant differences. There was no statistical difference in pH, PCO2, hemoglobin (Hb), and hematocrit (hCT) between the two groups. At the fourth week after surgery, the PO2 and SaO2 levels in the modeling group decreased, while Hb and hCT levels significantly increased, showing significant statistical differences compared to the control group; There was no difference in pH and PCO2 between the two groups.

　　6. Precautions: Surgical instruments should be strictly disinfected to prevent surgical infections, surgical trauma should be minimized, and aseptic operation should be strictly enforced.