动物造模,药效评价,心肌缺血 z-bio 2024-07-24 466

　　1. Modeling material animals: healthy dogs, weighing 10-20kg; Medications: Pentobarbital, ergometrine.

　　2. Modeling method: Animals were anesthetized intravenously with pentobarbital at a dose of 35mg/kg. A catheter was inserted from the right femoral artery into the main opening of the left coronary artery, and ergometrine at a dose of 0.22mg/kg (dissolved in 10ml of physiological saline) was injected within 1 minute.

　　3. The principle of modeling is that ergometrine causes myocardial ischemia in animals.

　　4. Changes after modeling: Injection can cause coronary artery spasm and myocardial ischemia in animals. The electrocardiogram can show T wave changes, followed by ST segment changes and a decrease in QRS wave voltage.

　　Pathological changes: Observation under light and transmission electron microscopy revealed that the main microvascular vessels in coronary arteries and myocardium were endothelial cell swelling, degeneration and shedding, exposed inner elastic membrane, uneven inner membrane surface, platelet and red blood cell adhesion on the elastic membrane in some lumens, microthrombus formation, and narrowing of lumens. The myocardial cells show vacuolar degeneration, enhanced eosinophilia, irregular sarcomeres, bamboo like changes in myofibrils, some exhibit contraction spasms, some have myofilament dissolution, edema, unclear sarcomeres, disordered arrangement of intercalated discs, mitochondrial swelling, cristae rupture, or even membrane rupture.