动物造模,药效评价,血管外膜炎症 z-bio 2024-07-26 334

　　1. Modeling material animal: New Zealand white rabbit with large ears, male or female, weighing 1.5-2.0kg.

　　2. The modeling method involves feeding a high-fat diet (regular basic feed+7% peanut oil+1.5% cholesterol) for two weeks before surgery. After anesthesia with 30mg/kg ketamine and 5mg/kg, the animals were disinfected and the skin at the groin area was cut open to expose the femoral artery. A polyethylene sleeve (1.5cm long, inner diameter 1.5mm2, outer diameter 2.0mm2) was placed over the femoral artery, and a medical cotton thread (1.5cm long) soaked overnight in 6mg/L lipopolysaccharide physiological saline was placed between the sleeve and the artery to ensure sufficient contact between the cotton thread and the outer membrane of the artery. The contralateral side was treated with physiological saline instead of 6mg/L lipopolysaccharide physiological saline. After surgery, all animals were still fed a high-fat diet.

　　3. Comparison of biochemical changes after modeling with the intima area of the control group: The intima area of the blood vessel was (0.71 ± 0.05) mm2 at week 0 after modeling and (0.75 ± 0.15) mm2 at week 2 after modeling.

　　4. Pathological changes after modeling: No inflammatory cell infiltration was observed in the vascular wall 0 days after modeling; Three days after surgery, significant inflammatory cell infiltration was observed on the outer membrane surface, and inflammatory cell adhesion was also observed on the vascular endothelium; At 2 weeks after operation, inflammatory cells in the adventitia were still seen infiltrating, mostly monocyte macrophages, and a small number of foreign body macrophages. In addition, smooth muscle cells in the middle membrane of the blood vessels showed foam, and intimal hyperplasia was seen. Sometimes, plaque appeared in the intima and protruded into the lumen.