动物造模,药效评价,脑缺血 z-bio 2024-07-26 418

　　1. Modeling material animals: Healthy male 2nd grade Kunming mice, weighing 12-22g; Medications: Pentobarbital, 2,3,5-triphenyltetrazolium chloride.

　　2. Modeling method: After anesthetizing mice with pentobarbital at a concentration of 20g/L and a dose of 60mg/kg, the neck skin was incised in the middle, and the left common carotid artery, external carotid artery, and internal carotid artery were separated under a microscope. The proximal end of the common carotid artery and external carotid artery were ligated with silk thread, and silk thread was pre placed at the internal carotid artery. The internal carotid artery was temporarily clamped with a microvascular clamp. Cut a small incision with a microvascular at approximately 1.0-2.0mm near the bifurcation of the common carotid artery, insert a pre prepared 8-0 nylon fiber segment, deliver it to the proximal end of the internal carotid artery, release the vascular clamp, and continue to push the segment into the starting part of the middle cerebral artery. Pre set the line at the site of ligating the internal carotid artery. During the operation, use a 37 ℃ constant temperature heating pad to keep warm. After the operation, place the mice in a 32 ℃ constant temperature incubator for 3 hours, and then feed them freely in a cage.

　　3. The principle of modeling is to ligate blood vessels, which leads to cerebral ischemia.

　　4. Changes after modeling: The volume of cerebral infarction in animals after modeling was (120 ± 17) mm3, and the degree of cerebral edema was (21.5 ± 2.5)%.