动物造模,药效评价,先天肾阳虚证 z-bio 2024-07-29 448

　　(1) Copy method: Adult mice, good at calling fierce cats. When raising mice to 75 days of age, they were mated in a 2:1 ratio between males and females. On the evening of the 4th day, male mice were taken out, and on the 5th day, the experimental mice were threatened by placing the pregnant mice directly under a cat cage. The cat cage was only separated from the mouse cage by a net, and the cat claws could touch the female mice. The mice were threatened for 2 hours in the morning and afternoon every day, for a total of 14 days, until 1 day before the pregnant mice gave birth. Feed 1-2 male or non experimental mice to the cat during each threat to increase the atmosphere of intimidation. On the 19th day after mating, female mice began to give birth one after another. Some newborn mice were euthanized and their testicles were taken for relevant examinations. The remaining animals were raised and euthanized for 75 days, and their testicles were taken for relevant examinations.

　　(2) Characteristics of the model: HE staining was performed on the right testis of male offspring mice, and it was observed that there was significant edema in the interstitium of spermatogenic cells. Some spermatogenic cells were loose and swollen in the cytoplasm, while others were accompanied by the formation of varying sizes of vacuoles in the cytoplasm. Sperm production decreased in the lumen of the seminiferous tubules, and the number of layers of spermatogenic cells showed an upward trend. The number of spermatogenic cells in the specimen increased significantly at 880 μ m3. The offspring mice were raised and euthanized until the 75th day. The ratio of testes to body weight of male mice born to threatened pregnant mice showed no significant abnormalities. When the left testicle was refrigerated for 15 days, it was found that there were differences in dryness, wetness, and color compared to normal mice, and the coefficient increased significantly. After freezing for 60 days, the testes of the offspring mice were soaked in 10% formaldehyde solution for 31 days. After slicing, HE staining was used to observe under a light microscope. The number of layers of spermatogenic cells in the seminiferous tubules was 4-5, and the arrangement of spermatogenic cells was irregular and unevenly distributed. There was obvious edema in the interstitium of spermatogenic cells, and most of the cytoplasm of spermatogenic cells had vacuolar degeneration, half of the perimeter was unclear, and some showed obvious decay. A small number of scattered macrophages appeared in the seminiferous tubules, and some formed multinucleated giant cells. Sperm production was significantly reduced and unevenly distributed in the lumen.

　　(3) Comparative medicine selects animals with significant weight loss within 3 days of birth based on the traditional Chinese medicine theory that newborns are weak and small, which belongs to fetal timidity and renal insufficiency. Alternatively, traditional Chinese medicine uses the fear of kidney injury theory to intimidate pregnant mice and cause congenital kidney deficiency in offspring. Individuals with fetal timidity have immature intrauterine development and insufficient innate endowments. It is characterized by delayed growth and development, low physiological function, and a syndrome of spleen and kidney deficiency, especially kidney deficiency. Research has found that the fetal timidity model exhibits endocrine disorders such as pituitary gland, thyroid gland, and adrenal gland, while the congenital kidney deficiency caused by intimidating pregnant mice exhibits behavioral abnormalities.