动物造模,药效评价,肾阴虚证 z-bio 2024-07-29 347

　　(1) Method of replication: Adult mice were subcutaneously injected with hydrocortisone at a dose of 1mg per mouse, once every other day, for a total of three times. Detect relevant indicators of fatigue resistance test, cold resistance test, hypoxia resistance test, carbon particle phagocytosis test, humoral immunity test and other experiments.

　　(2) The model features significantly reduced swimming time and hypoxia tolerance time of animals at room temperature; The cold resistance and carbon particle clearance index significantly decreased; The half hemolysis value (HC50) was significantly reduced, indicating a significant inhibitory effect on mouse specific antibody production induced by sheep red blood cells.

　　(3) There are two modeling methods for the comparative medical model of adrenal cortex hyperfunction and kidney yin deficiency syndrome: one is the adrenocorticotropic hormone modeling method, which involves excessive use of adrenocorticotropic hormone in the model animals to replicate the adrenal cortex hyperfunction model by promoting the synthesis and release of adrenocorticotropic hormone. Theoretical basis, mainly for patients with adrenal cortex hyperfunction, clinical manifestations are often of traditional Chinese medicine kidney yin deficiency syndrome type. This molding method has a short cycle and usually takes 4 days to successfully mold. The second is the adrenal cortex hormone modeling method, which directly overuses glucocorticoids to model animals, causing an increase in exogenous glucocorticoid levels and replicating a model similar to adrenal cortex hyperfunction. Commonly used drugs include hydrocortisone, prednisolone, hydrocortisone acetate, hydrocortisone acetate, hydrocortisone sodium succinate, dexamethasone sodium phosphate, dexamethasone, and other drugs. The molding cycle is 4-14 days.