动物造模,药效评价,肾阴虚证 z-bio 2024-07-29 370

　　(1) Method of replication: Adult rats were orally administered thyroid hormone at a dose of 2.5mg/kg body weight per day for 3 weeks. The changes in body weight, water intake, urine output, pain threshold, heart rate, etc. before and after modeling were routinely measured. At the end of modeling, blood was collected from the abdominal aorta to measure serum triiodothyronine (T3), thyroid hormone (T4), blood glucose, serum IgG, copper (Cu), zinc (Zn) element content, plasma viscosity, and the animals were euthanized. The thyroid gland, adrenal gland, and spleen were weighed for pathological examination.

　　(2) The characteristics of the model include weight loss, increased water intake, and decreased urine output in animals; Heart rate significantly increases and pain threshold decreases; Blood glucose and serum IgG levels decrease, while plasma viscosity increases significantly; The serum levels of Cu and Zn significantly increased, and the Cu/Zn ratio significantly increased; The levels of serum T3 and T4 significantly increased; Thyroid weight decreased, spleen weight did not increase, and there was no significant change in adrenal gland weight index. The cells in the zona glomerulosa of the adrenal cortex are slightly dense, with reduced thickness. Under the microscope, the cell volume is slightly increased and the cytoplasm is slightly enriched; The number of cells per unit area in the outer layer of the fasciculus decreased, and pathological section examination showed no significant changes in the thyroid, spleen, adrenal cortex reticular zone, and adrenal medulla.

　　(3) Comparative medicine kidney yin deficiency syndrome refers to the syndrome characterized by insufficient kidney yin and internal disturbance of deficiency fire. Its main clinical features include waist and knee pain, nocturnal emissions, menstrual disorders, and the presence of yin deficiency and internal heat. This type of model replicates an animal model of hyperthyroidism by administering high doses of thyroid hormone to experimental animals, resulting in an increase in exogenous thyroid hormone levels. The theoretical basis is mainly that patients with hyperthyroidism have a high basal metabolic rate, and their clinical manifestations can present a type of yin deficiency and internal heat similar to traditional Chinese medicine kidney yin deficiency syndrome. The available drugs include T3, T4, triiodothyronine suspension, thyroid tablets, thyroid hormone powder suspension, etc. The shortest molding cycle is 3 days, and the longest is 21 days. Generally, it takes 7-10 days to successfully mold.