动物造模,药效评价,细菌性支气管肺炎 z-bio 2024-07-31 420

　　1. Modeling material animals: Kunming mice, weighing 21-25g; Drug: Klebsiella pneumoniae strain, hydrocortisone, cyclophosphamide for injection.

　　2. Modeling method: The Klebsiella pneumoniae strain is diluted into a suspension of 1 × 10 ^ 7 CFU/ml after enrichment, toxicity enhancement, identification, and cultivation.

　　The modeling animals were injected intraperitoneally with 0.1ml (0.5mg) of hydrocortisone and 0.2ml (0.5mg) of cyclophosphamide every morning and afternoon, respectively, for a total of 3 days. After the last administration of cyclophosphamide 1-2 hours after immunosuppression in mice, a pediatric scalp needle was inserted into the upper right part of the anterior back, about 1cm from the right ear root (disinfected), and 0.1ml of bacterial solution was injected. Those who died within 5 hours after injection were considered non infectious and should be discarded.

　　3. Modeling principle: Klebsiella pneumoniae infection causes animal pneumonia.

　　4. Changes after modeling: After about 5-12 hours of infection, the modeling animals exhibit inactive movements, lack of appetite, slow response to the outside world, slight arching of the back, upright back hair, and subsequently limp limbs (with both hind limbs often extended backwards) and unable to support the body; When lung lesions are severe, the breathing surface is shallow and rapid; Finally, his breathing gradually weakened and he died.

　　Pathological examination shows varying degrees of bronchopneumonia, characterized by exudation and infiltration of inflammatory cells around the bronchioles and bronchioles, as well as within the alveoli. Interstitial capillary congestion or bleeding may occur, and the lesions may appear focal or patchy depending on the severity.

　　Compared with the control group (6.209 ± 1.037), the white blood cell count (x 10 ^ 9) of the modeling animals (2.220 ± 0.847) was significantly reduced.