动物造模,药效评价,移植性鼻咽癌 z-bio 2024-07-31 663

　　Transplanted nasopharyngeal carcinoma animal model refers to a model cultivated by transplanting human nasopharyngeal carcinoma tissue or cells into experimental animals. It is one of the most commonly used animal models for nasopharyngeal carcinoma in recent years.

　　The discovery and application of thymus deficient nude mice and severe combined immunodeficiency SCID mice in experimental animals have made the study of human tumor xenografts more convenient and feasible. Human tumor cells and tissues transplanted into nude mice and SCID mice can be passaged and grown, while their tumor cell morphology, chromosome number, and isoenzyme levels remain unchanged.

　　[Modeling Method] Select nasopharyngeal carcinoma cell lines with clear features and widely used, such as CNE-1, CNE-2, 5-8F-EGFP, etc. During the logarithmic growth phase of cells, digest the cells with trypsin and count them. After washing with PBS, prepare a cell suspension with a cell concentration of 10000000/ml in PBS. Select 4-week BALB/C-nu/nu nude mice and inject 0.2ml PBS cell suspension subcutaneously into the armpit or back using a 1ml syringe under sterile conditions. Observe the appearance time of tumor nodules at the injection site twice a week. Measure the size, weight, surface and central ulcer necrosis rate of the tumor. Human nasopharyngeal carcinoma biopsy tissue can also be inoculated into nude mice. After successful primary transplantation, tumor blocks can be taken and transplanted between nude mice.

　　[Model features] The transplantation of nasopharyngeal carcinoma cell lines into nude mice or SCID mice has a high tumor formation rate and is easy to observe. Tumor cells can maintain the histological and ultrastructural characteristics of human nasopharyngeal carcinoma. Generally, fresh tissue block transplantation has the highest tumor formation rate, short tumor incubation time, and short tumor volume doubling time. However, the size of the implanted tissue blocks is difficult to maintain consistency, and the volume after tumor formation is not uniform. The tumor formation rate of cell suspension inoculation is about 88%, and the incubation time of tumor formation is relatively long. However, the number of inoculated cells can be quantified, and the resulting tumor volume is uniform, which is suitable for experimental studies with high volume consistency requirements. Direct injection of 5-8F-EGFP strain with high metastatic activity into the nasopharynx of nude mice resulted in brain, lymph node, and lung metastasis.

　　Model evaluation and application: The formation ability of nasopharyngeal carcinoma tissue and cell xenografts in nude mice can evaluate the degree of malignant transformation and tumorigenicity of cells. The advantage of xenografts is that after inoculating a certain amount of tumor cells or cell-free filtrate (viral tumors), a group of nude mice can carry the same tumor, with consistent growth rates, small individual differences, high survival rates, and similar host effects. It is easy to objectively judge the efficacy, and can be continuously transplanted in the same or same strain of animals for long-term retention for experimental use. The experimental period is generally short, the operation is relatively simple, and the technology is mature. So, in the screening of anti nasopharyngeal carcinoma drugs, transplant tumor trials are mostly used. However, transplanted tumors have a fast growth rate, high proliferation rate, and short volume doubling time, which are significantly different from human nasopharyngeal carcinoma. The transplant tumor model is also used for research on the pathogenesis and prevention of nasopharyngeal carcinoma in humans. The in situ transplant tumor model can more objectively simulate the occurrence and development process of tumors in the human body than the subcutaneous transplant tumor model.