动物造模,药效评价,心肌缺血 z-bio 2024-07-31 431

　　(1) The replication method involves taking experimental dogs weighing approximately 30kg, administering intravenous phenobarbital sodium anesthesia at a dose of 35-40mg/kg body weight, and maintaining anesthesia at 4mg/(kg · h). The animal is fixed in the right lateral position, with tracheal intubation, positive pressure ventilation, and regulated by measuring end expiratory CO2 concentration and blood gas indicators. Insert two peripheral veins and administer anesthetics and experimental drugs separately. Continuously record the second lead electrocardiogram. Inject microspheres into the left coronary artery through an arterial angiography catheter, starting with 10ml and then 5ml after a 5-minute interval. Microsphere injection can gradually increase LVEDP (left ventricular end diastolic pressure). When LVEDP increases to 16-18mm Hg (2.1-2.4kPa) and/or MPAP (mean pulmonary artery pressure) increases to 20mmHg (2.7kPa) and/or heart rate reaches 200 beats per minute, stop the injection of embolic agents. By injecting microspheres with an average dose of 3-5 mg/kg, embolization is completed approximately 70 minutes later. It takes at least 30 minutes for hemodynamics to stabilize after coronary artery occlusion.

　　(2) Model characteristics When testing drugs that may improve heart function, this model can be used for hemodynamic detection. The model prepared by this method can be used to evaluate the effect of drugs on myocardial function in left heart failure caused by acute myocardial ischemia.

　　(3) In clinical comparative medicine, patients with hypertension and coronary atherosclerosis are induced by fatigue, excessive exercise, infection, fever, blood viscosity, coronary spasm, etc., which lead to insufficient blood supply to the myocardium and cause myocardial ischemia, which is a chronic progressive disease. tiny

　　The ball injection method directly blocks coronary blood vessels with small microspheres to cause acute blood supply deficiency, which has certain differences from the clinical pathogenesis process; But the symptoms, signs, and changes in electrocardiogram caused by it are similar to those in clinical practice.