动物造模,药效评价,心肌缺血 z-bio 2024-07-31 484

　　(1) The replication method was performed on experimental dogs weighing 10-15kg. After routine anesthesia, Seldinger technique was used to deliver self-made polyvinyl chloride constrictors (including effective stenosis length of 10mm) through the right femoral artery to the left circumflex branch (LCX) or left anterior descending branch (LAD) of the coronary artery, successfully establishing 50% or 75% stenosis of the coronary artery. Selective left coronary angiography, left ventricular angiography, and myocardial pathology examination were performed 5-10 months after surgery.

　　(2) Model features: ① The method is simple and easy to implement, with a high success rate and strong repeatability. ② The indicators for judging vascular stenosis and myocardial infarction are intuitive and reliable Simultaneously conducting research on hemodynamics, myocardial electrophysiology, coronary angiography, biochemical metabolism, pathophysiology, etc. is beneficial for clinical and basic research that combines function and morphology Due to the large area of myocardial damage, myocardial pathological anatomy, tissue trace elements, and local microcirculation can be studied simultaneously Experimental studies on chronic myocardial ischemia-reperfusion can be conducted.

　　(3) The comparative medical open chest method for preparing myocardial ischemia models requires open chest surgery and the use of ventilators to assist breathing, resulting in significant trauma and higher mortality rates in the model animals. The closed chest method is simple and easy to perform, with minimal surgical trauma and low animal mortality rate. The postoperative model animals recover quickly, and any coronary artery can be selected, with accurate positioning. Compared with the myocardial ischemia model caused by the open chest method, it is more in line with the pathological and physiological changes of clinical myocardial ischemia, and the animals are under less physiological disturbance. The myocardial ischemia model prepared by this method was used for experimental research, and the results were relatively accurate with small errors.