动物造模,药效评价,动脉血栓 z-bio 2024-07-31 399

　　(1) The replication method involves separating the common carotid artery and femoral artery on both sides of the experimental rabbit after routine anesthesia, and clamping the ends of one common carotid artery with two arteries at a distance of about 2.5cm between the clamps; Insert a No. 4 needle into the artery and fix it, quickly extract residual blood from the arterial segment, and rinse it three times with physiological saline until there is no residual blood in the arterial segment. Inject 0.4ml of 10% H2O2 solution into the arterial segment in 3-4 portions and continue to act for 10 minutes. Rinse it twice with physiological saline, then release the blood flow, and use medical adhesive to seal the needle hole to stop bleeding; Cut off the common carotid artery and weigh the thrombus segment after 2 hours.

　　(2) The model features fixed thrombus location and easy quantification of thrombus. The model is easy to replicate and has good reproducibility. The material is simple and easy to obtain.

　　(3) Numerous studies in comparative medicine have shown that reactive oxygen species can damage vascular tissue and endothelial cells. H2O2 is a type of reactive oxygen species that, like other free radicals, can also damage various tissues. In this experiment, a high concentration H2O2 solution was used to establish a rabbit carotid artery thrombosis model by damaging the vascular endothelium. The experiment proved that all rabbits had thrombosis formation. When the endothelium of rabbit arterial blood vessels is damaged with H2O2, platelets adhere to the exposed subendothelial tissue at the damaged vessel, and then aggregate, secrete, and release thromboxane A2 (TXA2), further activating platelets and activating the coagulation system. Thrombin promotes the formation of fibrin, ultimately forming a wall attached thrombus.